REGULAR CONTENT
Final ID
618
Type
Educational Exhibit-Poster Only
Authors
D Woods1, G Jacobs2, S Leonard1, A Ranjan3, J Esparza-Trujillo4, P Yarmolenko5, A Partanen6, I Bakhutashvili4, J Geschwind7, E Levy1, K Sharma8, M Dreher9, V Krishnasamy1, A Mikhail4, J Karanian4, W Pritchard4, B Wood10
Institutions
1NIH, Bethesda, MD, 2NIH, BETHESDA, MD, 3Oklahoma State University, Bethesda, MD, 4National Institutes of Health, Bethesda, MD, 5Childrens National Medical Center, Washington, DC, 6Philips, Washington, DC, 7Yale University School of Medicine, New Haven, CT, 8Childrens National Medical Center, Mc Lean, VA, 9Biocompatibles, Inc., Oxford, CT, 10National Institutes of Health, North Bethesda, MD
Purpose
To describe a step-wise method and practice for rabbit VX2 tumor model development, inoculation, propagation, and maintenance for interventional oncology research models.
Materials & Methods
A VX2 rabbit tumor model has been incompletely described. VX2 rabbit tumor cell maintenance propagation and inoculation are described in a recipe for interventional oncology research programs in New Zealand white rabbits. Cell suspension and image-guided inoculation techniques are described for donor and recipient animals with harvesting of aliquots from donors. Inoculation success rates were analyzed and fluoroscopic, cone beam CT, CT, and microCT imaging of tumors was performed in vivo or ex vivo.
Results
Tips and tricks for successful maintenance of a VX2 tumor model program are described. Specific tools and techniques for successful inoculation, harvest, and imaging are detailed. Expected successful inoculation rates are characterized along with techniques to minimize peritoneal tumor nontarget growth during intrahepatic inoculation including coaxial ultrasound guidance techniques with gel foam pledgets. Imaging features are described for liver VX2.
Conclusions
VX2 rabbit tumor model may provide a cost effective method for optimization and characterization of interventional oncology applications, models, and technology. Publication of difficulties and failures during model development may help others avoid similar problems. A recipe for success requires understanding the shortcomings, risks, and pitfalls of such model development.
Final ID
618
Type
Educational Exhibit-Poster Only
Authors
D Woods1, G Jacobs2, S Leonard1, A Ranjan3, J Esparza-Trujillo4, P Yarmolenko5, A Partanen6, I Bakhutashvili4, J Geschwind7, E Levy1, K Sharma8, M Dreher9, V Krishnasamy1, A Mikhail4, J Karanian4, W Pritchard4, B Wood10
Institutions
1NIH, Bethesda, MD, 2NIH, BETHESDA, MD, 3Oklahoma State University, Bethesda, MD, 4National Institutes of Health, Bethesda, MD, 5Childrens National Medical Center, Washington, DC, 6Philips, Washington, DC, 7Yale University School of Medicine, New Haven, CT, 8Childrens National Medical Center, Mc Lean, VA, 9Biocompatibles, Inc., Oxford, CT, 10National Institutes of Health, North Bethesda, MD
Purpose
To describe a step-wise method and practice for rabbit VX2 tumor model development, inoculation, propagation, and maintenance for interventional oncology research models.
Materials & Methods
A VX2 rabbit tumor model has been incompletely described. VX2 rabbit tumor cell maintenance propagation and inoculation are described in a recipe for interventional oncology research programs in New Zealand white rabbits. Cell suspension and image-guided inoculation techniques are described for donor and recipient animals with harvesting of aliquots from donors. Inoculation success rates were analyzed and fluoroscopic, cone beam CT, CT, and microCT imaging of tumors was performed in vivo or ex vivo.
Results
Tips and tricks for successful maintenance of a VX2 tumor model program are described. Specific tools and techniques for successful inoculation, harvest, and imaging are detailed. Expected successful inoculation rates are characterized along with techniques to minimize peritoneal tumor nontarget growth during intrahepatic inoculation including coaxial ultrasound guidance techniques with gel foam pledgets. Imaging features are described for liver VX2.
Conclusions
VX2 rabbit tumor model may provide a cost effective method for optimization and characterization of interventional oncology applications, models, and technology. Publication of difficulties and failures during model development may help others avoid similar problems. A recipe for success requires understanding the shortcomings, risks, and pitfalls of such model development.