REGULAR CONTENT
Final ID
419
Type
Original Scientific Research-Oral or Pos
Authors
W Park1, S Cho1, X Huang1, A Larson1, D Kim2
Institutions
1Northwestern University, Chicago, IL, 2Northwestern University/Department of Radiology, Chicago, IL
Purpose
Bacteriolytic therapy using anaerobic bacteria is considered as a promising approach for the treatment of solid tumors. To improve therapeutic efficacy while potentially reducing toxicity, we demonstrate an approach to label Clostridium novyi-NT (C. novyi-NT) spores with branched gold nanoparticles (BGNP) thus permitting intra-procedural X-ray CT visualization of spore delivery to solid tumors upon intra-tumoral injection.
Materials & Methods
C. Novyi-NT spores were kindly provided by BioMed Valley Discoveries (Kansas City, MO, USA). BGNP were synthesized in an aqueous solution of sodium cholate and then the BGNP were modified with 4-mercaptobenzoic acid to render negatively charge. BGNP-coated spores were prepared using a simple electrostatic deposition method. In vitro phantom and in vivo (PC-3 human prostate cancer mice model) CT studies were performed using a micro-CT imaging system (NanoPET/CT®, Mediso Ltd-Bioscan Inc., Hungary-US).
Results
Successful preparation of BGNP-coated C. Novyi-NT spores was confirmed by various physico-chemical characterization methods. In the PC3 mouse model, BGNP-coated spores were locally injected into the hypoxic center of each tumor guided with CT confirmation of intra-tumoral delivery. The therapeutic efficacy of these CT guided infused BGNP-coated spores demonstrated in immunohistochemical analyses such as H&E and TUNEL staining. The infused BGNP-coated spores exhibited strong therapeutic efficacy consistent with that observed for native spores suggesting that the innate anti-tumoral function was preserved.
Conclusions
Our BGNP-coating on the C. Novyi-NT spores have the potential to not only facilitate CT image-guided bacteriolytic procedures but also to serve as a novel BGNP-mediated combinational therapeutic delivery platform.
Final ID
419
Type
Original Scientific Research-Oral or Pos
Authors
W Park1, S Cho1, X Huang1, A Larson1, D Kim2
Institutions
1Northwestern University, Chicago, IL, 2Northwestern University/Department of Radiology, Chicago, IL
Purpose
Bacteriolytic therapy using anaerobic bacteria is considered as a promising approach for the treatment of solid tumors. To improve therapeutic efficacy while potentially reducing toxicity, we demonstrate an approach to label Clostridium novyi-NT (C. novyi-NT) spores with branched gold nanoparticles (BGNP) thus permitting intra-procedural X-ray CT visualization of spore delivery to solid tumors upon intra-tumoral injection.
Materials & Methods
C. Novyi-NT spores were kindly provided by BioMed Valley Discoveries (Kansas City, MO, USA). BGNP were synthesized in an aqueous solution of sodium cholate and then the BGNP were modified with 4-mercaptobenzoic acid to render negatively charge. BGNP-coated spores were prepared using a simple electrostatic deposition method. In vitro phantom and in vivo (PC-3 human prostate cancer mice model) CT studies were performed using a micro-CT imaging system (NanoPET/CT®, Mediso Ltd-Bioscan Inc., Hungary-US).
Results
Successful preparation of BGNP-coated C. Novyi-NT spores was confirmed by various physico-chemical characterization methods. In the PC3 mouse model, BGNP-coated spores were locally injected into the hypoxic center of each tumor guided with CT confirmation of intra-tumoral delivery. The therapeutic efficacy of these CT guided infused BGNP-coated spores demonstrated in immunohistochemical analyses such as H&E and TUNEL staining. The infused BGNP-coated spores exhibited strong therapeutic efficacy consistent with that observed for native spores suggesting that the innate anti-tumoral function was preserved.
Conclusions
Our BGNP-coating on the C. Novyi-NT spores have the potential to not only facilitate CT image-guided bacteriolytic procedures but also to serve as a novel BGNP-mediated combinational therapeutic delivery platform.